Dibutyl phthalate (DBP) is commonly used to increase the exibility of plastics\r\nin industrial products. However, several plasticizers have been illegally used as clouding\r\nagents to increase dispersion of aqueous matrix in beverages. This study thus develops a\r\nrapid and validated analytical method by ultra-performance liquid chromatography with\r\ntandem mass spectrometry (UPLC-MS/MS) for the evaluation of pharmacokinetics of DBP\r\nin free moving rats. The UPLC-MS/MS system equipped with positive electrospray\r\nionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor\r\nm/z 279.25148.93 transitions for DBP. The limit of quantication for DBP in rat plasma\r\nand feces was 0.05 �µg/mL and 0.125 �µg/g, respectively. The pharmacokinetic results\r\ndemonstrate that DBP appeared to have a two-compartment model in the rats; the area\r\nunder concentration versus time (AUC) was 57.8 �± 5.93 min g/mL and the distribution\r\nand elimination half-life (t1/2, and t1/2,) were 5.77 �± 1.14 and 217 �± 131 min, respectively,\r\nafter DBP administration (30 mg/kg, i.v.). About 0.18% of the administered dose was\r\nrecovered from the feces within 48 h. The pharmacokinetic behavior demonstrated that\r\nDBP was quickly degraded within 2 h, suggesting a rapid metabolism low fecal cumulative\r\nexcretion in the rat.
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